Research Articles
2023
Transfer RNA mediated restoration of potassium conductance and electrical correction in premature termination Long-QT Syndrome hERG mutants. Blomquist VG,* Niu J, Choudhury P, Al Saneh A, Colecraft HM#, Ahern CA. Molecular Therapy . Accepted 9/16/2023.
* Co-first author, Ahern lab, # Co-corresponding
Beta-subunit-eliminated eHAP expression (BeHAPe) cells reveal subunit regulation of the cardiac voltage-gated sodium channel. Minard AY, Clark CJ*, Ahern CA#, Piper RC. Journal of Biological Chemistry . 2023 Aug 4:105132. doi: 10.1016/j.jbc.2023.105132. PMID: 37544648
* Co-first author, Ahern lab MSTP, # Co-corresponding
Homomeric interactions of the MPZ Ig domain and their relation to Charcot-Marie-Tooth disease. . Ptak CP, Peterson TA, Hopkins JB, Ahern CA, Shy ME, Piper RC. Brain. August, awad258, https://doi.org/10.1093/brain/awad258
# -provided know-how and reagents.
Phospholipase C-ε defines a PACAP-stimulated pathway for secretion in the chromaffin cell. Chen X, Coffman BL, Brindley RL, Galpin JD, Ahern CA, Currie KPM, Smrcka AV, Axelrod D, Anantharam A. J Neuroendocrinol. 2023 Mar 12:e13255. doi: 10.1111/jne.13255. Online ahead of print PMID: 36970756
Mechanosensitive pore opening of a prokaryotic voltage-gated sodium channel. Strege PR, Cowan LM, Alcaino C, Mazzone A, Ahern CA, Milescu LS, Farrugia G, Beyder A. Elife 2023 Mar 13;12:e79271. doi: 10.7554/eLife.79271. PMID: 36970756
Optically derived bases for CFTR phosphoregulation. Infield DT, Schene ME, Fazan FS, Galles GD, Galpin JD, Ahern CA. Journal of General Physiology. 2023 Apr 3;155(4):e202213216. doi: 10.1085/jgp.202213216. Epub 2023 Jan 25. PMID: 36912788
Chemically Acylated tRNAs are Functional in Zebrafish Embryos. Brown W, Galpin JD, Rosenblum C, Tsang M, Ahern CA#, Deiters A. Journal of the American Chemical Society. 2023 Jan 20. doi: 10.1021/jacs.2c11452. Online ahead of print. PMID: 36669466. #-Provided chemical reagents for the project.
Tuning phenylalanine fluorination to assess aromatic contributions to protein function and stability in cells. Galles GD, Infield DT, Clark CJ, Hemshorn ML, Manikandan S, Fazan F, Rasouli A, Tajkhorshid E, Galpin JD, Cooley RB, Mehl RA, Ahern CA. Nature Communications. 2023 Jan 4;14(1):59. doi: 10.1038/s41467-022-35761-w. PMID: 36599844 Free PMC article.
2022
Backbone amides are determinants of Cl- selectivity in CLC ion channels. Leisle L, Lam K, Dehghani-Ghahnaviyeh S, Fortea E, Galpin JD, Ahern CA#, Tajkhorshid E, Accardi A. Nature Communications. 2022 Dec 6;13(1):7508. doi: 10.1038/s41467-022-35279-1. PMID: 36473856 Free PMC article. #-Provided chemical reagents for the project.
Role of a conserved ion-binding site tyrosine in ion selectivity of the Na+/K+ pump. Spontarelli K, Infield DT, Nielsen HN, Holm R, Young VC, Galpin JD, Ahern CA#, Vilsen B, Artigas P. Journal of General Physiology. 2022 Jul 4;154(7):e202113039. doi: 10.1085/jgp.202113039. Epub 2022 Jun 3. PMID: 35657726 Free PMC article. #-Provided chemical reagents for the project.
2021
Selection and validation of orthogonal tRNA/synthetase pairs for the encoding of unnatural amino acids across kingdoms. Galles GD, Infield DT, Mehl RA, Ahern CA. JMethods in Enzymology. 2021;654:3-18. doi: 10.1016/bs.mie.2021.03.009. Epub 2021 Apr 9. PMID: 34120719
HIFs: New arginine mimic inhibitors of the Hv1 channel with improved VSD-ligand interactions. Zhao C, Hong L, Galpin JD, Riahi S, Lim VT, Webster PD, Tobias DJ, Ahern CA#, Tombola F. Journal of General Physiology. 2021 Sep 6;153(9):e202012832. doi:10.1085/jgp.202012832. Epub 2021 July PMID: 34228044 Free PMC article. #-Provided chemical reagents for the project.
Cation-π Interactions and their Functional Roles in Membrane Proteins. Infield DT, Rasouli A, Galles GD, Chipot C, Tajkhorshid E, Ahern CA. Journal of Molecular Biology. 2021 Aug 20;433(17):167035. doi: 10.1016/j.jmb.2021.167035. Epub 2021 May 4.PMID: 33957146 Review.
2020
A rationally designed orthogonal synthetase for genetically encoded fluorescent amino acids. Steinberg X, Galpin J, Nasir G, Sepúlveda RV, Ladron de Guevara E, Gonzalez-Nilo F, Islas LD, Ahern CA#, Brauchi SE. Heliyon. 2020 Oct 7;6(10):e05140. doi: 10.1016/j.heliyon.2020.e05140. eCollection 2020 Oct. PMID: 33083608. #-Provided chemical reagents for the project.
Divergent Cl- and H+ pathways underlie transport coupling and gating in CLC exchangers and channels. Leisle L, Xu Y, Fortea E, Lee S, Galpin JD, Vien M, Ahern CA#, Accardi A, Bernèche S. Elife. 2020 Apr 28;9:e51224. doi: 10.7554/eLife.51224. PMID: 32343228 #-Provided chemical reagents for the project.
SCN2A channelopathies in the autism spectrum of neuropsychiatric disorders: a role for pluripotent stem cells? Kruth KA, Grisolano TM, Ahern CA, Williams AJ. Molecular Autism. 2020 Apr 7;11(1):23. doi: 10.1186/s13229-020-00330-9. PMID: 32264956
2019
Petersson EJ, Mehl RA, Ahern CA. Expanding Genetic Code Expansion through Resource Facilities, Workshops, and Conferences. International Journal of Molecular Science. 2019 Apr 29;20(9). pii: E2103. doi: 10.3390/ijms20092103. Conference Summary.
Lueck JD, Yoon JS, Perales-Puchalt A, Mackey AL, Infield DT, Behlke M5, Pope MR, Weiner DB, Skach WR, McCray PB Jr, Ahern CA. (2019) Engineered transfer RNAs for suppression of premature termination codons. Nature communications. 2019 Feb 18;10(1):822. PMID: 30778053
Clairfeuille T, Cloake A, Infield DT, Llongueras JP, Arthur CP, Li ZR, Jian Y, Martin-Eauclaire MF, Bougis PE, Ciferri C, Ahern CA, Bosmans F, Hackos DH, Rohou A, Payandeh J. (2019) Structural basis of α-scorpion toxin action on Nav channels. Science. 2019 Feb 7. pii: eaav8573. PMID: 30733386
2018
Infield DT, Matulef K, Galpin JD, Lam K, Tajkhorshid E, Ahern CA, Valiyaveetil FI.(2018) Main-chain mutagenesis reveals intrahelical coupling in an ion channel voltage-sensor. Nature communications. 2018; 9(1):5055. PMID: 30498243
Infield DT, Lee EEL, Galpin JD, Galles GD, Bezanilla F, and Ahern, CA. (2018) Replacing voltage sensor arginines with citrulline provides mechanistic insight into charge versus shape. The Journal of general physiology. 150(7):1017-1024. PMID: 29866793
Molinarolo S, Lee S, Leisle L, Lueck JD, Granata D, Carnevale V, and Ahern, CA. (2018) Cross-kingdom auxiliary subunit modulation of a voltage-gated sodium channel. The Journal of biological chemistry. 2018; 293(14):4981-4992. PMID: 29371400
Thompson A, Infield DT, Smith AR, Smith GT, Ahern CA, Zakon HH. (2018) Rapid evolution of a voltage-gated sodium channel gene in a lineage of electric fish leads to a persistent sodium current. PLoS biology.2018; 16(3):e2004892. PMID: 29584718
Infield DT, Lueck JD, Galpin JD, Galles GD, and Ahern, CA. (2018) Orthogonality of Pyrrolysine tRNA in the Xenopus oocyte. Scientific reports. 2018; 8(1):5166. PMID: 29581437
2016
Leisle L, Chadda R, Lueck JD, Infield DT, Galpin JD, Krishnamani V, Robertson JL, Ahern, C.A. (2016) Cellular encoding of Cy dyes for single-molecule imaging. eLife. 2016 Dec 12;5. pii: e19088. doi: 10.7554/eLife.19088. PMID: 27938668
Lueck, JD, Mackey, AL., Infield, DT., Li, J., Roux, B., and Ahern, C.A. (2016) Atomic mutagenesis in ion channels with engineered stoichiometry. eLife. Oct 6;5. pii: e18976. doi: 10.7554/eLife.18976. PMID: 27710770
2015 and beyond
Kim RY, Yau MC, Galpin JD, Seebohm G, Ahern C.A., Pless SA, Kurata HT. (2015) Atomic basis for the therapeutic activation of neuronal potassium channels. Nature Communications. Sep 3;6:8116. doi: 10.1038/ncomms9116.PMID: 26333338
Pless, SA., Galpin, JG., Niciforovic, A. Nunez, J.J, Kurata, H.A and Ahern, C.A. (2013) Hydrogen bonds as molecular timers for slow inactivation in voltage-gated potassium channels. eLife. Dec 10;2:e01289. doi: 10.7554/eLife.01289. PMID: 24327560
Pless, SA., Galpin, JG., Niciforovic, A. Nunez, J.J, Kurata, H.A and Ahern, C.A. (2013) A novel mechanism for fine-tuning open state stability in a voltage-gated potassium channel. Nature Communications. 4:1784. doi: 10.1038/ncomms2761.PMID: 23653196
Pless, SA., Galpin, JG., Frankel, A., and Ahern, C.A. Molecular Basis for Class Ib Anti-Arrhythmic Inhibition of Cardiac Sodium Channels. (2011) Nature Communications, 2:351. doi: 10.1038/ncomms1351.PMID: 21673672
Pless, SA., Galpin, JG., Niciforovic, A. and Ahern, C.A. (2011) Contributions of Counter-Charge in a Potassium Channel Voltage-Sensing Domain. Nature Chemical Biology,7(9):617-23. Cover Article PMID: 21785425
Reviews and Book Chapters
Molinarolo S, Granata D, Carnevale V, and Ahern, CA. (2018) Mining Protein Evolution for Insights into Mechanisms of Voltage-Dependent Sodium Channel Auxiliary Subunits. Handbook of experimental pharmacology. 2018; 246:33-49. PMID: 29464397
Ahern, C.A, Payandeh, J., Bosmans, F., and Chanda, B. (2016) A hitchhikers guide to the sodium channel galaxy. Journal of General Physiology. 147(1):1-24. doi: 10.1085/jgp.201511492. Review. PMID: 26712848
Leisle L, Valiyaveetil F, Mehl RA, Ahern C.A. (2015) Incorporation of noncanonical amino acids. Adv. Exp Med Biol. 2015;869:119-51. doi: 10.1007/978-1-4939-2845-3_7. Chapter in the Springer book: Applying Chemical Biology to Ion Channels.
Corry, B., Lee, S., and Ahern C.A. (2014) Pharmacological Insights and Quirks of Bacterial Sodium Channels. Book chapter, P.C. Ruben (ed.), Voltage Gated Sodium Channels, Handbook of Experimental Pharmacology 221, DOI 10.1007/978-3-642-41588-3. PMID: 24737240
Pless S.A, Kim, R.Y. Ahern, C.A., and Kurata H.T. (2015) Atom-by-Atom Engineering of Voltage-Gated Ion Channels: Magnified Insights into Function and Pharmacology, Journal of Physiology. doi: 10.1113/jphysiol.2014.287714. PMID:25640301
Pless S.A and Ahern C.A. (2013) Unnatural Amino Acids as Probes of Ligand-Receptor Interactions and Their Conformational Consequences. Annual Review of Pharmacology and Toxicology. 53:211-29. PMID: 23294309
